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KMID : 0364820080440020156
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Korean Journal of Microbiology
2008 Volume.44 No. 2 p.156 ~ p.163
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Biodegradation of Kerosene by Pseudomonas aeruginosa K14
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Kim Jee-Young
Lee Sang-Seob
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Abstract
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In this study, we isolated 32 strains of kerosene degrading bacteria from oil contaminated soil by enrichment culture.
Isolates were screened for kerosene degradation efficiencies and K14 were selected which had the highest
removal efficiency for 1,000 mg/L of kerosene. K14 were identified as Pseudomonas aeruginosa by morphological,
biochemical test and 16S rDNA analysis. The optimal culture condition were determined as initial
inoculated cell concentration, 1.0 g/L; substrate concentration, 1,000 mg/L; temperature 30oC; pH 7. When we
enforced batch test in this condition, K14 degraded 72% of kerosene with 1,000 mg/L during 72 hr. And, at low
concentration (200 mg/L), K14 degraded 95.8% of kerosene during 48 hr. As a result, kerosene biodegradation
by Pseudomonas aeruginosa K14 could be useful for clean up of groundwater and soil contaminated with crude
oil.
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KEYWORD
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biodegradation, kerosene, Pseudomonas aeruginosa
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